Journal: Nature Communications

Article Title: Living sensor display implanted on skin for long-term biomarker monitoring

doi: 10.1038/s41467-025-67384-2

Figure Lengend Snippet: a Bright-field images of the engrafted tissue-engineered skin before LPS stimulation, more than 2 months post-transplantation. b Fluorescence images of the engrafted tissue-engineered skin before LPS administration, c 2 days after the intraperitoneal administration of LPS, d 7 days after administration, e Fluorescence image of the engrafted tissue-engineered skin at 2 days after subcutaneous administration of LPS on the 9th day post-initial administration. f Time course of fluorescence intensity calculated from the image analysis of the tissue-engineered skin. The vertical dotted lines indicate the time points of fluorescence observation, and the downward‑pointing triangles indicate the timing of LPS administration. g Comparison of fluorescence intensities at 7 days and 9 days after administration. Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.00000056. The statistical analysis was performed using t-tests (two-tailed). *** p < 0.001. h Comparison of fluorescence intensities at 2 days and 7 days after administration. The statistical analysis was performed using t-tests (two-tailed). Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.3898 i Immunostaining image merged for human mitochondria and GFP from a skin section taken from around the engrafted skin at 9 days after LPS administration. Representative of three independent experiments with similar results. j Fluorescence image confirming the localisation of inflammatory cells positive for Ly6g and F4/80 in the magnified area. Representative of three independent experiments with similar results.

Article Snippet: Following the removal of the BSA solution, the tissue sections were incubated with the diluted primary antibody: Cytokeratin 14 Polyclonal antibody (Proteintech・10143-1-AP,CK14), Anti-Collagen IV antibody(abcam・ab6586, Col4),Vimentin (D21H3) XP Rabbit mAb (CST 5741S, Vimentin), Anti-GFP (Rat IgG2a) Monoclonal (GF090R) CC (nacalali,04404-84,GFP), Anti-GFP Antibody (abcam・ab290, GFP), Purified Rat Anti-Mouse CD31 (BD pharmingen・553370, CD31), Anti-Mitochondria antibody (Merck (Sigma)・MAB1273, Human Mitochondria), Rat Anti-Mouse F4/80 antibody (Bio-Rad・MCA497GA, F4/80), Anti-CD86 antibody (Cell Signalling Technology・ #19589S, CD86) Anti-CD163 antibody (abcam・ab182422, CD163), Anti-Ly6g antibody (abcam・ab25377, Ly6g), overnight at 4 °C within the moisture chamber.

Techniques: Transplantation Assay, Fluorescence, Comparison, Two Tailed Test, Immunostaining

Journal: Frontiers in Immunology

Article Title: Visualizing early allograft rejection: an M1 macrophage-specific GLUT1 probe predicts TCMR onset in renal transplantation

doi: 10.3389/fimmu.2025.1670370

Figure Lengend Snippet: Early detection of M1 macrophages is of significant importance for early warning of T-cell recruitment and diagnosis of TCMR. (A) Hematoxylin and Eosin (H&E) staining of renal allograft cortex. (B) H&E staining of renal allograft medulla. (C) T cell recruitment in renal allograft. (D) Single-cell sequencing reveals massive infiltration of M1 macrophages in early post-transplantation period. (E) Temporal dynamics of the M1 macrophage-specific inflammatory effector gene set during the early post-transplantation phase. (F) Communication between M1 macrophages and T cells in renal allograft. (G) Immunohistochemical staining for anti-CD86 validates M1 macrophage infiltration in renal allograft. ****:P<0.0001.

Article Snippet: M1 macrophages were incubated with probe XJYZ and APC-conjugated anti-mouse CD86 antibody (84393, Cell Signaling Technology) for 1 hour at 4°C in the dark.

Techniques: Biomarker Discovery, Staining, Sequencing, Transplantation Assay, Immunohistochemical staining